| CEMOVIS: Cryo-electron
microscopy of vitreous sections COURSE INFORMATION |
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Course Title: |
CEMOVIS: Cryo-electron microscopy of vitreous sections | |
| Institute: | Dept. of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden | |
| Course leader: | Sergej Masich | |
| Initial target
group: Experienced electron microscopists planning to implement CEMOVIS in their laboratory. |
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| Course level: | Advanced | |
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Course Objectives: |
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| Entry Level: Experienced electron microscopists (preference will be given to persons experienced in ultramicrotomy and in cryo-EM of vitreous specimen or in Tokuyasu's cryo-sections) |
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| Registration | Before September 31, 2008 with letter of motivation and CV. Preference will be given to suitable applicants who were rejected from the 2007 course solely due to space limitations. |
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| Location: | CMB, Karolinska Institutet, Stockholm, Sweden | |
| Length: | 5 days | |
| Starting days: | Sunday, November 23 (registration at 14.00) – Thursday, November 27 (departure after 16.00) | |
| Practice hrs: | 27 Hrs. | |
| Theory hrs: | 6 Hrs. | |
| Number of participants: | 8 | Min. 4, Max, 9 |
| Course material: | Students will be provided with a set of known high-quality specimens to ensure that course objectives are met; however, students wishing to bring their own, non-pathogenic materials may do so, provided that suitable arrangements have been made with the course leader at least 3 weeks prior to commencement of the course. | |
| Certification: | Yes | |
| Examination: | No | |
| Contents: | Theoretical knowledge - Ice, amorphous solid water and vitrification - High-pressure freezing - Cryo-ultramicrotomy of vitreous material - Observation in the cryo-EM - Basic practice with vitreous water - Specimen preparation - High-pressure freezing - Cryo-ultramicrotomy - Cryo-electron microscopy |
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| Systems used: | FEI, Leica , Diatome | |
| Fee: | None (travel and lodging at student's charge) | |
| Information: | Via course organizer: Sergej Masich | |
Remarks: |
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